Effect of the linear aliphatic amine functionalization on in vitro transfection efficiency of chitosan nanoparticles


Gok M. K. , Demir K. , Cevher E., Ozgumus S. K. , Pabuccuoglu S.

CARBOHYDRATE POLYMERS, cilt.207, ss.580-587, 2019 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 207
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.carbpol.2018.12.013
  • Dergi Adı: CARBOHYDRATE POLYMERS
  • Sayfa Sayıları: ss.580-587

Özet

The aim of this study is to prepare the long linear aliphatic amine pendant group-functionalized chitosan based nanoparticulate gene carrier system with improved properties for the efficient transfection. The amine functionalized chitosan (MChi) was synthesized by using N-(2-hydroxyethyl) ethylenediamine (HE-EDA) and characterized for the first time. The nanoparticles of MChi (nMChi) were prepared by ionic gelation method, and their particle size, polydispersity (PDI), zeta potential (mV), gene binding capacity and cytotoxicity were determined. Green Fluorescent Protein circular plasmid DNA (pEGFN1) loaded nanoparticles (gnMChi) were used in the transfection studies. The results showed that nMChi with a particle size of 102.9 nm and zeta potential of 41.9 +/- 5.63 mV was non-toxic, had high transfection efficiency in Human Embryonic Kidney 293 and Primary Ovine Fibroblast cell lines and would be used as an efficient gene carrier system.

The aim of this study is to prepare the long linear aliphatic amine pendant group-functionalized chitosan basednanoparticulate gene carrier system with improved properties for the efficient transfection. The amine func-tionalized chitosan (MChi) was synthesized by using N-(2-hydroxyethyl)ethylenediamine (HE-EDA) and char-acterized for thefirst time. The nanoparticles of MChi (nMChi) were prepared by ionic gelation method, andtheir particle size, polydispersity (PDI), zeta potential (mV), gene binding capacity and cytotoxicity were de-termined. Green Fluorescent Protein circular plasmid DNA (pEGFN1) loaded nanoparticles (gnMChi) were usedin the transfection studies. The results showed that nMChi with a particle size of 102.9 nm and zeta potential of41.9 ± 5.63 mV was non-toxic, had high transfection efficiency in Human Embryonic Kidney 293 and PrimaryOvine Fibroblast cell lines and would be used as an efficient gene carrier system.