Due to the fact that they produce less post-operative pain and shorter hospitalisation times, laparoscopic surgery procedures are more widely used than many traditional open surgical methods. However, the carbon dioxide gas used to visualise the surgical site during the procedure causes complications such as an increase in the blood CO2 level and acidosis. In the present study, 12 male pigs were used. Sedation was achieved via intramuscular injection of xylazine at a dose of 2 mg/kg and anaesthesia induction was done via slow intravenous injection of ketamine HCl at a dose of 2 mg/kg. Following endotracheal intubation, general anaesthesia was maintained using isoflurane. Blood samples were collected from the retrobulbar plexus immediately before CO2 pneumoperitoneum (T-0) and 30 min aft er insufflation (T-1). Cases were evaluated with respect to pH, pCO(2), HCO3-, and SpO(2) levels. The findings obtained revealed that there was a statistically significant difference between T-0 and T-1 measurement times with respect to pCO(2) and HCO3- although there were no significant differences between the other evaluated parameters. As a result, it has been demonstrated that carbon dioxide pneumoperitoneum in laparoscopic surgery increases blood CO2 levels. Careful anaesthesia, endotracheal intubation, and 100% O-2 inhalation throughout anaesthesia, however, can compensate for this increase. Consequently, with the preservation of blood pH and the stabilisation of pO(2) levels, laparoscopic interventions can be performed without endangering the patient's life.