Studies on the in vitro maturation of cat oocytes


Evecen M. , Atalla H., Birler S., Baran A., Ak K.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.27, sa.6, ss.1409-1412, 2003 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 27 Konu: 6
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0093-691x(02)01235-9
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Sayfa Sayıları: ss.1409-1412

Özet

The aim of the present study was to investigate the effects of gonadotrophin and Bovine Serum Albumin (BSA) supplemented Synthetic Oviduct Fluid (SOF) and Ham's F-10 media on the in vitro maturation of cat oocytes. Cocytes collected from spayed stray queens served as the material of the study. The ovaries were brought to the laboratory within 2 h in PBS solution at 38 degreesC. Recovered oocytes were divided into two groups (Group 1: SOF + 0,4% BSA + 10 mug/ml FSH + 10 mug/ml LH, group 2: Ham's F-10 + 0,4% BSA + 10 mug/ml FSH + 10 mug/ml LH) and left for maturation in an incubator at 38.5 degreesC for 48 h under atmosphere containing 5% O-2, 5% CO2 and 90% N-2 and almost 100% humidity. Oocytes were then fixed and stained. The maturation status of the oocytes was evaluated under a phase-contrast microscope at x400 magnification. Data were compared by using Student's t-test. Group 1 had 209 oocytes and group 2 had 207: a total of 416. In group 1 54.06% (113/209) and in group 2 29.00% (60/207) of the oocytes reached the M II stage. The difference between these values was statistically significant. At the end of the study, it was concluded that SOF medium was superior to Ham's F-10 medium for the in vitro maturation of cat oocytes. These results also showed that a satisfactory background for in vitro fertilization studies of cat oocytes has been established.