In this research, the objective is to determine the level of knowledge, attitudes and behaviors of university students on the matter of sexual health. The research was descriptive and cross-sectional. The sample is comprised of the students from the A degrees stanbul University BakA +/- rkoy School of Health and Hasan Ali Yucel Faculty of Education (N = 877). The survey form used for data collection includes questions on socio-demographic features, knowledge, attitudes and behaviors of students on sexual health. In analyzing the data, averages and percentages are calculated and Chi-square tests are performed. The average age of the students in our study was 20.7 +/- A 1.718 years and only 39.0% of them had sufficient knowledge on sexuality. Analysis revealed that there were differences between men and women about their reported relationship with the opposite sex (p < 0.001), premarital sexual intercourse (p < 0.001), expectation of virginity from spouse (p < 0.001) and sexual preference (p < 0.001) were significant. We have concluded that university students' sexual knowledge is insufficient, their behaviors and attitudes are influenced by traditional approaches and understanding of sexuality differs between two genders.