In this study, the rate of nasal carriage of S.aureus in hospital staff and oxacillin resistance in S.aureus ln strains were investigated. Conventional methods were used for the isolation and susceptibility. From 300 nasal swabs cultured, 100 (33.3%) S.aureus strains were isolated and 9 (9 %) of them were found to be oxacillin resistant. The isolates of S.aureus were found to be resistant to penicillin, ampicillin - sulbactam, cefazolin, clindamycin, eritromycin, tetracycline, gentamicin, trimethoprim-sulfamethoxazole, rifampin, ciprofloxacin, norfloxacin, ceftriaxone, imipenem and mupirocin in the rates of 93%, 5%, 7%, 9%, 11%, 20%, 6%, 5%, 4%, 1%, 3%, 2%, 1%, 2%, respectively. All the strains were found to be sensitive to vancomycin and teicoplanin. These results showed that oxacillin resistance is not an important problem in our hospital staff: However surveys should continue and precautions should be taken.