A four-year-old cross-breed and neutered bitch weighing 24 kg, was presented with vaginal bleeding for one month and a vaginal mass identified two previously. An ovariohysterectomy operation was performed at two years of age; however, mating subsequently continued. Vaginal bleeding and an irregular-shaped, oedematous vaginal mass (12 x 9 x 7 cm) were detected on clinical examination. The bitch also exhibited leukocytosis and erythrocytopenia. Abundant erythrocytes together with neutrophils, lymphocytes, intermedier cells and transmissible venereal tumour cells were observed in vaginal cytology. Abdominal ultrasonography showed cystic ovarian tissue (2.42 x 1.36 cm) next to the right kidney. Chemotherapy was performed once a week for six weeks leading to a gradual declined in the size and oedema of the mass and to a stop in bleeding. Subsequently, the vaginal mass was extirpated and the ovarian remnant tissue was removed via laparotomy on the same day. The bitch was found to be healthy on examination four months later.