Pinus nigra subsp. pallasiana var. yaltirikiana has a very restricted spread, and is an endemic black pine variety in Turkey. This plant has a poor generative reproduction performance. It is unclear which factors are responsible for this low reproduction capacity. Therefore, objective of this study was to investigate pollen and seed characteristics of the plant. For this purpose, pollen samples were collected in the pollination period, and seed samples were collected in the cone ripening period in the natural distribution site of this plant in Black Sea Region. Pollen samples were examined whether or not they have normal shape and size. Seed samples were tested for germination percentage and seed size and soundness were also examined. Results showed that pollen samples seemed to have normal shape and size, and seeds have a germination percentage of 71. This study indicated that the conservation of Pinus nigra subsp. pallasiana var. yaltirikiana through in-situ and ex-situ strategies and the establishment of a seed orchard in a different area by taking grafts are important in terms of ensuring continuity of limited number individuals.