Forensic scientists often use nuclear STR loci for identification. But sometimes it is difficult to get successful results from nuclear DNA, especially when the DNA in a sample is degraded or the amount is not sufficient. In these cases forensic scientists use mitochondrial DNA (mtDNA). The aim of this work is to genotype of the 9 mitochondrial SNP sites (3010, 5004, 6776, 8592, 10394, 10754, 11864, 15340, 16519) and to identify polymorphic sites within these SNP's in Turk population, while validating a routine procedure that is optimized to analyze samples trace amount of DNA. After optimization, blood samples from 30 volunteers and trace amount of DNA containing samples from 5 of these volunteers were analyzed successfully except hanky with mucus on it. These samples were hair, nails, earrings, toothbrushes, qtip, glass edge swabs, gum, razors and cigarette butts. The result of 30 volunteers showed that the SNP sites 3010 and 16519 were polymorphic sites among Turks, and the 15340. SNP site was different than the rCRS (revised Cambridge Reference Sequence) at all volunteers. © 2011 Elsevier Ireland Ltd.