Corynebacterium pseudotuberculosis is an important animal pathogen with a broad host spectrum that can cause clinical diseases. Notably, this bacterium is a facultative intracellular organism that causes caseous lymphadenitis. C. pseudotuberculosis infection was reported in many animals, although there have been no reports of isolation from amphibians. In this report, an abscess on the tibiofemoral joint of a Budgett's frog (Lepidobatrachus laevis) housed in a private zoo in Istanbul, Turkey was investigated. A sample of purulent material from the abscess was cultured, and a pure growth of C. pseudotuberculosis was identified by a commercial biochemical test system (API-Coryne, BioMerieux, France) and confirmed by the BD-Phoenix Automated Microbiology System, while nucleotide sequences analysis was performed for molecular identification. As a result, the organism was confirmed as C. pseudotuberculosis. Treatment with enrofloxacin was empirically started, and the bacteria were confirmed to be sensitive to enrofloxacin. Thereafter, 0.2% nitrofurantoin pomade was injected into the wound. Complete healing was evident following treatment. This report showed, for the first time, the isolation of C. pseudotuberculosis from a frog. The possible theory for the aetiology of this disease is feeder insects, which likely spread the bacteria into the enclosure of the frog. This suggests that invertebrates may act as a reservoir of infection and that the inter-species colonisation was be able to increase over time. Due to the zoonotic characteristics of the agent, an increase in the effect of colonisation was considered important for human health.