THE FAT PAT TISSUE EXPRESSES A PATHOGENIC PROFILE OF INFLAMMATORY MEDIATORIES AND 4-HNE IN PATIENTS WITH OSTEOARTHRITIS


Tanrıverdi B., Tanrıverdi G. , Dağıstanlı F.

Acta Medica Mediterranea, cilt.37, sa.2, ss.1045-1051, 2021 (SCI Expanded İndekslerine Giren Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 37 Konu: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.19193/0393-6384_2021_2_160
  • Dergi Adı: Acta Medica Mediterranea
  • Sayfa Sayıları: ss.1045-1051

Özet

Introduction: In this study we aimed to estimate if there is a releation between some inflammatory or adipocyte-derived cytokines such as IL-1 beta, IL-6, TNF-alfa, VEGF, 4-HNE, macrophages, mast cells and osteoarthirites. Especially 4-HNE which associated with obesity is a new marker for estimating the chondral injuries and there is a poor evidence of it’s effect on IFP and osteoarthritis. Methods: We obtained tissues after ethical approving and designed our studying groups as one osteoarthritis (n=15) and one non-osteoarthritis patients group (n=12) and then applied histological procedure for estimating the IFP tissues. We stained using IL1beta, IL-6, TNF-alfa, VEGF, 4-HNE, CD68 (for macrophage marker) antibodies by immunohistochemical technique and toluidine blue for the mast cells, examined slides microscopically and evaluated all of the tissues according to the staining intensity by using HSCORE. Results: As a result, we detected high expression levels of inflammatory mediators especially IL-6, IL-1beta, VEGF and 4-HNE in the osteoarthritis group (OAG) than the non-osteoarthritis group (NOAG) and the numbers of macrophages and mast cells are higher in the OAG, too. Also, the macrophages have distinctly different morphologies in the OAG and they were bigger and had crown like structures compare to the NOAG. Conclusions : In conclusion, these data indicate that IFP-derived inflamatory mediators could contribute to pathophysiological processes in the osteoarthritis of the knee joint and there is a necessity for future studies in this area.