Introduction: In this study we aimed to estimate if there is a releation between some inflammatory or adipocyte-derived cytokines
such as IL-1 beta, IL-6, TNF-alfa, VEGF, 4-HNE, macrophages, mast cells and osteoarthirites. Especially 4-HNE which associated
with obesity is a new marker for estimating the chondral injuries and there is a poor evidence of it’s effect on IFP and osteoarthritis.
Methods: We obtained tissues after ethical approving and designed our studying groups as one osteoarthritis (n=15) and one
non-osteoarthritis patients group (n=12) and then applied histological procedure for estimating the IFP tissues. We stained using IL1beta, IL-6, TNF-alfa, VEGF, 4-HNE, CD68 (for macrophage marker) antibodies by immunohistochemical technique and toluidine
blue for the mast cells, examined slides microscopically and evaluated all of the tissues according to the staining intensity by using
Results: As a result, we detected high expression levels of inflammatory mediators especially IL-6, IL-1beta, VEGF and 4-HNE
in the osteoarthritis group (OAG) than the non-osteoarthritis group (NOAG) and the numbers of macrophages and mast cells are
higher in the OAG, too. Also, the macrophages have distinctly different morphologies in the OAG and they were bigger and had crown
like structures compare to the NOAG.
Conclusions : In conclusion, these data indicate that IFP-derived inflamatory mediators could contribute to pathophysiological
processes in the osteoarthritis of the knee joint and there is a necessity for future studies in this area.