A Novel Partial Sequence Alignment Tool for Finding Large Deletions


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Aruk T., Ustek D., Kursun O.

SCIENTIFIC WORLD JOURNAL, ss.1-6, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası:
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1100/2012/694813
  • Dergi Adı: SCIENTIFIC WORLD JOURNAL
  • Sayfa Sayıları: ss.1-6

Özet

Finding large deletions in genome sequences has become increasingly more useful in bioinformatics, such as in clinical research and diagnosis. Although there are a number of publically available next generation sequencing mapping and sequence alignment programs, these software packages do not correctly align fragments containing deletions larger than one kb. We present a fast alignment software package, BinaryPartialAlign, that can be used by wet lab scientists to find long structural variations in their experiments. For BinaryPartialAlign, we make use of the Smith-Waterman (SW) algorithm with a binary-search-based approach for alignment with large gaps that we called partial alignment. BinaryPartialAlign implementation is compared with other straightforward applications of SW. Simulation results on mtDNA fragments demonstrate the effectiveness (runtime and accuracy) of the proposed method.