The present study was designed to determine genotypes of adipocyte determination and differentiation-dependent factor 1 (ADD1)/sterol regulatory element-binding protein-1c (SREBP1c) in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle. Fifty cattle from the SAR breed and 43 cattle from the EAR breed were used. Intron 7 of the ADD1/SREBP1c gene containing an 84 bp indel mutation was amplified by polymerase chain reaction (PCR). The number of SS genotyped individuals with the 84 bp deletion associated with fatty acid composition and growth traits were very low in both breeds. The frequency of the LL genotype with the 84 bp insertion was significantly higher. The 2 cattle populations were in Hardy-Weinberg equilibrium. The SS genotype was observed in the SAR and EAR breeds. As a result, we can conclude that determination of ADD1/SREBP1c genotype distribution in a great number of cattle breeds of Bos taurus and Bos indicus would allow us to observe more SS genotypes.