Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum beta-lactamases


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Vahaboglu H., Ozturk R. , Akbal H., Saribas S. , Tansel O., Coskunkan F.

JOURNAL OF CLINICAL MICROBIOLOGY, cilt.36, ss.827-829, 1998 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 36 Konu: 3
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1128/jcm.36.3.827-829.1998
  • Dergi Adı: JOURNAL OF CLINICAL MICROBIOLOGY
  • Sayfa Sayısı: ss.827-829

Özet

A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of bla(OXA) was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.