Salmonella Spp. and Shigella Spp. detection via multiplex real-time PCR and discrimination via MALDI-TOF MS in different animal raw milk samples


Demirci M., Yigin A., Altun S. K. , Uysal H. K. , Saribas S., Kocazeybek B. S.

NIGERIAN JOURNAL OF CLINICAL PRACTICE, cilt.22, ss.1083-1090, 2019 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 22 Konu: 8
  • Basım Tarihi: 2019
  • Doi Numarası: 10.4103/njcp.njcp_596_18
  • Dergi Adı: NIGERIAN JOURNAL OF CLINICAL PRACTICE
  • Sayfa Sayısı: ss.1083-1090

Özet

Aims: The aim of this study was to provide epidemiological data about the presence of Salmonella spp. and Shigella spp. in raw milk samples collected from different animals. Methods: A total of 231 raw milk samples from 48 cows, 65 goats, 65 sheep, and 53 donkeys were studied. The ISO 6579:2002 and ISO 21567:2004 methods, antimicrobial susceptibility tests, and serotyping were performed. Species and subspecies discriminations were made via matrix-assisted laser desorption/ionization-time of flight mass spectrometry. After DNA isolation from all samples, Salmonella spp. and Shigella spp. were detected using real-time polymerase chain reaction (PCR) kits. Results: Five samples (2.16%) showed positivity out of 231 raw milk samples for Salmonella spp., and 2 (0.87%) samples were detected to be positive by multiplex real-time PCR design. Conclusion: We found that raw milk samples were not free of Salmonella spp. and Shigella spp. and need to be tested routinely to avoid public health problems. Rapid and reliable real-time PCR method can be developed and used for this purposes instead of slow bacterial culture processes.