Molecular and pathological investigations of EHV-1 and EHV-4 infections in horses in Turkey


Turan N. , Yildirim F. , Altan E., Sennazli G. , Gurel A., Diallo I., et al.

RESEARCH IN VETERINARY SCIENCE, cilt.93, ss.1504-1507, 2012 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 93 Konu: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.rvsc.2012.01.019
  • Dergi Adı: RESEARCH IN VETERINARY SCIENCE
  • Sayfa Sayısı: ss.1504-1507

Özet

The aim of the present study was to investigate abortion storms that occurred in the Marmara region of Turkey in 2008-2009 using a real-time PCR. Two aborted foetuses were necropsied and histo-pathological findings reported herein. Ten lungs, 3 brains and one nasal swab from 10 aborted foetuses, 6 nasal swabs and 3 vaginal swabs from aborting mares were included in this study. EHV-1 was isolated from the lung, liver and brain of 1 aborted foetus. EHV-1 DNA was detected in the lungs, livers and spleens of 2 necropsied foetuses and in 3 lungs from 10 foetuses submitted for diagnosis. A brain from one of the aborted foetuses was also positive for EHV-1 DNA. EHV-4 DNA was detected only in a nasal swab of one of the tested foetuses. Neither EHV-1 nor EHV-4 DNA was detected in the swabs of aborting mares. Sequence analysis of the glycoprotein B of the strains was performed and a phylogenetic tree was generated. The results indicated that 4 of the 5 Turkish EHV-1 strains (TR02, TR03, TR04 and TR05) clustered together; the fifth strain (TR01) was slightly removed from the group and clustered with other EHV-1 from various origins. Single nucleotide polyporphism (SNP in ORF30) associated with neuropathogenesis was not detected in any of the strains.