CHEMISTRY AND ECOLOGY, cilt.27, ss.31-41, 2011 (SCI İndekslerine Giren Dergi)
The aim of this investigation was to examine biochemical differences in the gastropod Rapana venosa (R. venosa) from polluted (RvP) and nonpolluted (RvN) sites of the Black Sea's Bulgarian coast that may serve as bioindicators of environmental quality. Mussels Mytilus galloprovincialis (MMg) were collected from polluted (MMgP) and nonpolluted (MMgN) sites at the same time for comparison. Bioactive compounds and the antioxidant activity of dry matter (DM) methanol extracts from RvP and RvN were determined. Three-dimensional fluorometry (3D-FL) and Fourier transform infrared (FTIR) analyses were used to evaluate polyphenols and proteins. DM methanol extracts from RvP, and to lesser extent RvN, showed high amounts of total phenolics (23.22 +/- 2.9 and 15.60 +/- 1.8mgGAE center dot g-1DW) and exhibited high levels of antioxidant activity in some radical scavenging assays ( MTE center dot g-1DW): 56.38 +/- 5.2 and 33.79 +/- 3.3 by 2,2azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) diamonium salt (ABTS center dot+); 54.79 +/- 5.6 and 33.7 +/- 2.6 by cupric reducing antioxidant capacity (CUPRAC); 62.34 +/- 6.8 and 30.31 +/- 3.9 by the 1diphenyl-2-picrylhydrazyl method (DPPH). A correlation in the above indices was found between R. venosa and M. galloprovincialis, but all results were relatively higher for M. galloprovincialis than for R. venosa. The obtained indices of R. venosa may serve as a bioindicator of environmental ecological quality.