Voltammetric Sensing of Uremic Toxin Indoxyl Sulfate Using High Performance Disposable Screen-Printed Graphene Electrode


Filik H. , Avan A. A. , Aydar S.

CURRENT PHARMACEUTICAL ANALYSIS, cilt.12, ss.36-42, 2016 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 12 Konu: 1
  • Basım Tarihi: 2016
  • Doi Numarası: 10.2174/1573412911666150520000605
  • Dergi Adı: CURRENT PHARMACEUTICAL ANALYSIS
  • Sayfa Sayısı: ss.36-42

Özet

We focused on a rapid method for the assay of protein unbound indoxyl sulfate (IS) in urine samples by square-wave voltammetry. A sensor based on the electroactivity of commercial graphene screen-printed electrode (GR-SPE) for sensitive estimation of uremic toxin indoxyl sulfate was presented. The behaviors of uremic solute indoxyl sulfate on GR-SP electrodes were explored by cyclic and square-wave voltammetry. The outcomes displayed that the GR-SP electrode exposed perfect electroactivity to uremic toxin indoxyl sulfate and the oxidation of uremic solute IS is a multi-step and irreversible process. The process is pH dependent and takes place with the forging of a main oxidation product that is adsorbed on the GR-SP electrode surface. The calibration curve was achieved in the IS concentration range of 0.5-80 mu M and the limit of detection was calculated to be 0.064 mu M at a signal-to-noise ratio of 3. The use of the commercially disposable GR-SPE in human serum and urine sample assays obtained satisfactory results, which made it a promising alternative in routine sensing applications.