Questioning the intestinal aganglionosis model by using benzalkonium chloride Benzalkonium klorid ile oluşturulan intestinal aganglionozis modelinin irdelenmesi

Gündoǧdu G., Polat E., Oktay R., Eliçevik M. , Tekant G. A. , Dervişoǧlu S., ...Daha Fazla

Cocuk Cerrahisi Dergisi, cilt.20, sa.3, ss.132-137, 2006 (Diğer Kurumların Hakemli Dergileri) identifier

  • Cilt numarası: 20 Konu: 3
  • Basım Tarihi: 2006
  • Dergi Adı: Cocuk Cerrahisi Dergisi
  • Sayfa Sayıları: ss.132-137


Aim: Aim of this study is questioning the validity of experimental aganglionosis producing procedure (EAPP) with benzalkonium chloride and reviewing the literature. Material and Methods: Study was designed with 10 rats in each 3 group; as late period (LP-1), late period control group (LP-2) and early changes in aganglionosis (EP). Also effects of long segment aganglionosis procedure were observed in 4 rats. To evaluate anatomical changes, opaque enemas were done pre-and post operatively. Ganglion changes were evaluated histopathologically. Clinical findings were observed. Results: LP-1 animals developed a functional obstruction clinic in early period but these findings resolved. There were no anatomical changes in opaque enemas except one animal. LP-1 animals lost weight during the procedure (p<0.05). Biopsy specimens showed normal cell count distribution, ganglion cells in LP-1. No clinical or histopathological changes were observed at control group of late period. In EP group, degeneration and decrease in ganglion cells were encountered. 4 animals which had a long segment aganglionosis procedure died due to massive ileus. Conclusion: In our study, our findings did not correlate with previous supporting studies. Due to literature search, we think that this temporary aganglionosis clinic occurs by degeneration in ganglions in early period but by neuronal plasticity phenomena; new ganglions occur and the aganglionosis clinic resolves. Thus EAPP leads to temporary aganglionosis and can not be a real model of Hirschsprung disease.