A 10 years old, intact, nulliparus, 7 kg in body weight, mixed bred bitch was presented to our clinic with vaginal mass, various sized tumoral masses on mammary tissue and anorexia. Pyometra and cystic structures on both ovaries were observed by ultrasonographic examination. As a surgical approach, ovariohysterectomy, bilateral mastectomy and extirpation of vaginal mass were applied in the same general anesthesia. Pathologic examination revealed several follicular cysts on both ovaries. In histological sections of the uterus, lumen intense neutrophil leukocytes in and pus which was formed by degenerated epithelia confirmed the presence of pyometra. It was identified histopathologically that the vaginal mass was a leiomyoma and the masses on mammary tissues were Adenocarcinoma grade II.