A 25 kg, 4 years old, Boxer breed bitch which had ovariohysterectomy section 2 years ago, was presented with weakness, loss of appetite, masses on abdominal and inguinal mammary glands and vaginal bleeding. Transmissible venereal tumor (TVT) was diagnosed with vaginal cytology. Intra-abdominal mass around the iliac artery and remnant ovary behind the right kidney were determined on ultrasonographic examination. The remnant ovary and the masses on mammary glands were removed by surgical approach. However, intra-abdominal mass could not be extirpated because of its localization during the same laparotomy. A biopsy was taken from the vaginal mass. According to histopathology, TVT and collagenous hamartoma were detected on mammary glands. Also, TVT was observed in vaginal and intra-abdominal masses. Chemotherapy protocol was arranged for intra-abdominal mass and vaginal TVT which occupied for ten weeks. Hemogram, vaginal smear and transabdominal ultrasonography were repeated before each chemotherapy course. Size of the intra-abdominal mass, pulsatility index (PI) and relative index (RI) values of iliac artery were evaluated with B-mode ultrasonography and doppler ultrasonography during the treatment, respectively. As a result, positive correlation between PI and RI values were determined. In the presented case, TVT and its metastases were successfully treated with both surgery and chemotherapy. It was concluded that evaluation of the metastases with doppler ultrasonography will be favorable to reveal the hemodynamic alterations of related organs or tissues.