Effect of ELF-EMF on K562 cell differentiation in the presence or absence of quercetin and heat-shock

Garip-Inhan A., Isal-Tugut I., Kalkan M. T.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.21, sa.2, ss.182-185, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 21 Konu: 2
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1080/13102818.2007.10817441
  • Sayfa Sayıları: ss.182-185


Extremely low frequency electromagnetic fields (ELF-EMF) are known to produce a variety of biological effects although the underlying mechanism has not been elucidated yet. The present study aimed to investigate the effect of ELF-EMF on K562 cells induced to differentiate with or without quercetin with application of heat-shock. 50Hz ELF-EW was applied at three (1mT,5mT,10mT) field intensities. Quercetin is,flavonoid known to have anti-tumorigenic and anti-proliferative properties. Heat-shock was applied since it has been shown that quercetin down-regulates but ELF-EMF increases heat-shock protein levels. Results of this study show that ELF-EMF increases differentiation of untreated K562 cells at low field intensities in accordance with the "window" effect of these fields. Heat-shock abolishes this increase. Heat-shock also decreases the %differentiation of quercetin induced cells. However it has no effect on quercetin induced ELF-EMF exposed cells implying a protective effect of ELF-EMF These results prompted us to hypothesize that these fields can act antagonistically with heat-shock under certain conditions.