Protective effect of hydrogen sulfide on experimental testicular ischemia reperfusion in rats


Bozkurt M., Degirmentepe R. B. , Polat E. C. , Yildirim F. , Sonmez K. , ÇEKMEN M. B. , ...Daha Fazla

Journal of Pediatric Urology, cilt.16, sa.1, 2020 (SCI Expanded İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Konu: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.jpurol.2019.10.006
  • Dergi Adı: Journal of Pediatric Urology

Özet

© 2019 Journal of Pediatric Urology CompanyAim: Testicular torsion is an urgent urological condition. Ischemia-reperfusion (I/R) processes that occur after detorsion as a treatment for torsion are caused by testicular injury. The purpose of our study is investigating the protecting effect of hydrogen sulfide (H2S) on the testicular ischemia reperfusion injury. Materials and methods: Thirty-eight Wistar-Albino rats were divided randomly into 6 different groups: Control (6); sham (6); IR-E (6)—2 h of torsion and 4 h of reperfusion; IR-E + H2S (6)—in addition to the IR-E group, 75 μmol/kg of sodium hydrogen sulfide (NaHS) was administered intraperitoneally 30 min before reperfusion; IR-L (7)—2 h of torsion and 24 h of reperfusion; IR-L + H2S (7)—in addition to the IR-L group, 75 μmol/kg NaHS was administered intraperitoneally 30 min before reperfusion. Biochemically, nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), reductive glutathione (GSH), and tumor TNF-α levels were measured in the testis. Serum TNF-α levels were also measured. Hematoxylin and eosin (H & E) was used for histopathological staining and microscopic findings were examined. The Johnsen score was performed to assess spermatogenesis activity in the testis. Apoptosis protease activating factor-1 (Apaf-1) and inducible nitric oxide synthase (iNOS) activity were evaluated immunohistochemically as well. Statistical analyses were made by the Chi-squared test and one-way analysis of variance. Results: MDA and NO levels were significantly increased in the IR-L group compared with sham and which decreased by the addition of H2S treatment to the IR-L group (p < 0.05) in biochemical evaluation. GSH vs SOD levels were decreased in the IR-L group compared with sham and which increased by the addition of H2S treatment to the IR-L group, but this correlations were not statistically significant (p > 0.05). Tissue and serum TNF-α levels were significantly increased in the IR-E group compared with sham and which decreased by the addition of H2S treatment to the IR-E group. Johnsen score was the lowest in IR-L group (p < 0.05). Apaf-1 and iNOS activity were significantly increased in the IR-L group compared with sham and which decreased by the addition of H2S treatment to the IR-L group (p < 0.05) in immunohistochemical evaluation. Conclusions: First, the authors would like to say that H2S treatment is protective and it is against ischemia reperfusion injury in testicular torsion. The anti-inflammatory, antioxidant, and antiapoptotic properties of H2S caused protective effect as shown in this study.[Formula presented]