Investigation of alcohol and benzodiazepines in injured traffic accidents with an optimized GC-MS method


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Doğusan Gökçe N., Mercan S. , Yeniocak S., Türkmen Z. , Söğüt Ö., Koldaş M., ...Daha Fazla

Australian Journal Of Forensic Sciences, cilt.111, ss.1-15, 2021 (SCI Expanded İndekslerine Giren Dergi)

  • Cilt numarası: 111
  • Basım Tarihi: 2021
  • Dergi Adı: Australian Journal Of Forensic Sciences
  • Sayfa Sayıları: ss.1-15

Özet

Benzodiazepines are frequently abused drugs due to their effects on central nervous system and these substances may cause impaired driving as well as other criminal cases.

The aim of this study was to determine the frequency of diazepam, alprazolam and clonazepam among traffic accidents besides alcohol. It was also aimed to develop an optimized GC-MS method for benzodiazepine analysis in serum samples. Biological samples were collected from 44 injured drivers involved in traffic accidents. For alcohol, whole blood samples were analyzed by Head Space/GC-MS.

The linearity range was found 1.25-30 μg/mL for diazepam, 5-120 for alprazolam, 10-60 for clonazepam and 10-60 for clonazepam-TMS. Calibration curves were acquired in linear form with R2 ≥0.999 correlation coefficients. The recovery ratios were found 70.2% and above.

Limit of Detection limits for diazepam, alprazolam, clonazepam and clonazepam-TMS were 0.396 μg/mL, 0.887 μg/mL, 3.472 μg/mL and 4.885 μg/mL respectively. Diazepam, alprazolam or clonazepam substances were not found in any of the serum samples while 16.2% of drivers were drunk.

As a conclusion, a validated analytical method was developed for determination of the most abused benzodiazepines in Turkey, which impair driving ability; it is planning to conduct for further studies with more cases.