Objective: Todetermine serum kisspeptin and anti-mullerian hormone (AMH) levels in women with and without polycystic ovary syndrome (PCOS). Materials and methods This was a prospective, cross-sectional, comparative study that included 70 women with PCOS and 58 non PCOS controls. PCOS patients were diagnosed according to the Rotterdam criteria. Age, body mass index (BMI), number of menstrual cycles per year, and the Ferriman-Gallwey Score were determined for each woman. Serum levels of kisspeptin, follicle stimulating hormone (FSH), luteinizing hormone (LH), thyroid stimulating hormone (TSH), estradiol, total testosterone, dehydroepiandrosterone sulfate(DHEA-S), AMH, fasting glucose and insulin were determined. Results Women with PCOS were younger (p < .001), with higher BMI (p = .027) and glucose values (p < .001); while displaying less number of menstrual cycles per year (p < .001). Although serum kisspeptin levels were similar in both groups, age was negatively (r= -0.33,p = .00018) and serum AMH levels were positively correlated (r = 0.25,p = .0039) with the serum kisspeptin levels in women with the PCOS. After adjusting for age, serum kisspeptin levels were comparable in both groups (p > .05). Serum LH, AMH, DHEA-S and total testosterone glucose, insulin levels and HOMA-IR values were significantly higher in women with PCOS as compared to controls (allp < .05). Conclusions Serum kisspeptin levels were similar in women with and without PCOS but positively correlated with AMH serum levels in PCOS women.