Molecular analysis based on partial B2L gene of Orf virus strains from human cases in Gaziantep region, Turkey

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Kuşkucu M. A.

ECCMID 2014, Amsterdam, Hollanda, 8 - 11 Mayıs 2014, ss.323

  • Basıldığı Şehir: Amsterdam
  • Basıldığı Ülke: Hollanda
  • Sayfa Sayıları: ss.323


Objectives: Gaziantep is located close to Turkey-Syria border where uncontrolled trans-boundary human and animal movements have been increased. Orf disease is a zoonotic infection caused by a dermotropic parapoxvirus. Human infections usually develop following occupational exposures. In 2012 a nosocomial outbreak occurred in a burn unit shortly after Eid al-Adha in Gaziantep affecting patients both of Turkish and Syrian origins. Also sporadic orf cases are encountered trough out the year with a peak in the period of Eid al-Adha in this region, who are usually cared by dermatology clinics. This year a burn patient, Syrian origin, developed orf infection again. In this study we analysed partial B2L region of the strains from the patients admitted in 2013 and compared with those strain from the nosocomial outbreak in 2012. Methods: Swab specimens of dermatological lesions were obtained from 1 burn unit patient and 6 dermatology patients. Partial B2L region amplified by nested polymerase chain reaction (PCR) and sequenced bi-directionally. All obtained sequences analysed by Molecular Evolutionary Genetics Analysis 5.1 software. Neighbour-joining phylogenetic tree with 1,000 replicates using Kimura-2 parameter distance matrix used for analysis. Results: Sequences of the strains from burned patient and dermatology patients were clustered in different lineages. All but 1 strains of 2013 were clustered with the strains from the outbreak in 2012. One strain belonging a dermatology patient was more closely related with the human and animal strains previously reported from Turkey. Conclusion: The data of our study and reported previously reported from Turkey indicate that different orf virus linages are circulating in the country. Interregional and/or trans-boundary human and animal movements may be one of the reasons of the diversity of orf virus strains. According to our results, different orf virus strains may circulate in even the same region.