© 2020 by TürkiyeKlinikleri.Objective: The aim of this study is to evaluate the microsurgical anatomy of human brain frontal lobe association white matter pathways (course, start and target zones), relationships with each other and its potential functional role with reference to clinical trials in the literature. Material and Methods: This study was performed between June 2013-August 2014 at Istanbul University Cerrahpaşa Faculty of Medicine, Microsurgery-Neuroanatomy Laboratory of Department of Neurosurgery. Ten postmortem human brain specimens were prepared in accordance with Klingler’s method. Brain hemispheres were dissected step by step from lateral to medial and medial to lateral under operating microscope and images were captured at every stage. Results: As a results of the white matter fiber dissection step by step, white matter association pathways associated with frontal lobe or short white matter tracts in frontal lobe were shown to correlate clinical studies known in the literature. Conclusion: Knowledge of the anatomofunctional role of fiber tracts helps to understand pre-, intra-and postoperative clinical “disconnection syndromes” and white matter tumoral invasion patterns, and may improve presurgical planning and surgical strategy, thereby potentially decreasing surgical morbidity.