The diagnostic value of Western blot method in patients with cystic echinococcosis


Aslan M., Yuksel P. , Polat E. , Cakan H. , Ergin S. , Oner Y. A. , et al.

NEW MICROBIOLOGICA, cilt.34, ss.173-177, 2011 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 34 Konu: 2
  • Basım Tarihi: 2011
  • Dergi Adı: NEW MICROBIOLOGICA
  • Sayfa Sayısı: ss.173-177

Özet

Cystic echinococcosis (CE) is the larval cystic stage (called echinococcal cysts) of a small taeniid-type tapeworm (Echinococcus granulosus). Carnivores such as dogs are usually definitive hosts. Intermediate hosts are typically herbivores such as sheep and cattle. CE can be detected using various imaging techniques such as ultrasonography or radiology Moreover the primary diagnosis has to be confirmed by serological tests since the clinical signs of the disease are non-specific. This study examined the antigenic band patterns useful for serologic diagnosis of hydatidosis. We also report on the post-operative evolution of patients treated for this disease and also determined the diagnostic performance of Western blot IgG kit. Twenty-five (16 females and 9 males) non-operated patients with hydatid cysts (NOP) and 33 (21 females and 12 males) operated patients with hydatid cysts (OP) were included as study group and 22 healthy individuals (14 females and 8 males) with no known chronic diseases were included as a control group. The ages of the patients and control group individuals were between 16-83 years. Patient and control groups were matched for age and sex. Cyst hydatid IgG antibodies were detected in the sera from all patient groups but no antibodies were found in the sera from the control group using ELISA IgG method. Twenty-three (92%) non-operated patients and 18 (54.5%) operated patients exhibited positive results when Western blot IgG kit was used. The P7 band pattern was detected in the sera from all operated and non-operated patients. Twenty-seven of these positive cases had p7 and (p7+p16/18), (p7+p24/26) or (p7+p16/18+p24126). No antibodies against p7, p16/18 ye p24/26 band patterns were seen in sera from the control group A statistically significant difference was detected between operated and non-operated patients for Western blot positivity.(p<0.01). p: 0.018-X2=5,604- OR: 0.176- 95% CI: 0.037- 0.841.