Objective: To investigate the difference of first trimester (11-14 weeks) serum levels of metastin, chitotriosidase activity and UtA Doppler findings between women who subsequently develop preeclampsia and controls. Material and Methods: Study was designed as a retrospective case-control study of propectively collected data. Pregnancies that were followed at our clinic had UtA Doopler examination and serum samples were collected during 11-14 weeks screening. 30 healthy pregnant women and 31 women with preeclampsia that delivered during September 2008-May 2010 were included in the study. Maternal serum metastin, chitotriosidase and UtA Doppler findings were compared. Results: Mean maternal serum metastin (1554±385 pmol/L vs 1995±375 pmol/L, p=0.000) levels were significantly lower in women who subsequently developed preeclampsia than controls. Mean maternal serum chitotriosidase activity (681.6±248.3 nmol/mL/h vs 527.7±223.1 nmol/mL/h, p=0.014) and UtA PI (1.93±0.40 vs 1.27±0.35, p=0.000) was significantly higher in women who subsequently developed preeclampsia than controls. The areas under the curve equal to 0.896, 0.797 and 0.681 (p=0.000, p=0.000 and p=0.015) for UtA PI, metastin, and chitotriosidase respectively were determined for the prediction of preeclampsia. Conclusion: First trimester maternal serum metastin levels, chitotriosidase activity and UtA PI values are altered in women destined to become preeclamptic. UtA Doppler and metastin, have a potential to be used as a first trimester prediction test for preeclampsia. Copyright © 2012 by Türkiye Klinikleri.