4 th International VETIstanbul Group Congress, Almati, Kazakistan, 11 - 13 Mayıs 2017, ss.32
Trakya Bölgesindeki Sığır ve Abort Olmuş Sığır Fötuslarında Neospora caninum’un ELİSA, Histopatoloji, İmmunohistokimya ve PCR Yöntemleriyle Teşhisi.
DETECTION OF NEOSPORA CANINUM IN ABORTED BOVINE FETUSES AND DAM BLOOD SAMPLES BY ELISA, HISTOPATHOLOGY, IMMUNOHISTOCHEMISTRY AND PCR IN TRAKYA REGION, TURKEY.
Erdogan Bamac O.1*, Haktanir D.1, Cetinkaya H.2, Yuzbasioglu Ozturk G.1, Arun S.S.1, Gurel A.1
Istanbul University, Veterinary Faculty, Department of Pathology1, Istanbul University, Veterinary Faculty, Department of Parasitology2
Neospora caninum is one of the most major causal agent of abortion in cattle in many countries and it has a huge economic impact. In this study, it is aimed to detect the presence of N. caninum in the aborted bovine fetuses and dam blood samples by using diagnostic tools such as Enzyme-Linked ImmunoSorbent Assay (ELİSA), histopathology, immunohistochemistry (IHC) and Polymerase Chain Reaction (PCR) in Trakya Region, Turkey. The blood samples and the aborted fetuses of dairy cattle were collected and carried to the laboratuary with cold chain and after obtaining sera from the blood samples they were preserved at -20 C°. Following the necropsy of the fetuses, tissue sampleswere taken from the cerebrum, cerebellum, medulla spinalis, heart, liver, lung, kidney and umbilical cord and put into buffered formaldehyde solution for histological examination. In addition to this, 20 g of each organ was put into tubes of 1.5 ml for DNA extraction and PCR method and was preserved first at -80 C°and then at -20C°. Histopathological examinations revealed encephalitis in the brains of 9 fetuses (4.5%), myocarditisin the hearts of 12 fetuses (6%), pneumonia in the lungs of 26 fetuses (13%) and nephritis in the the kidneys of 4 fetuses (2%). PCR revealed positive results in the organs of 5 fetuses (2.5%). ELISA results supported PCR results however revealed higher positivity than PCR(4%). Immunohistochemistry detected infections in 5 fetuses only giving positive reactions in the brain and in the heart (2.5%).
Histopathological, serological, immunuhistochemical andPCR methods were found to be correlative and complementary.