Investigation of the in vitro cytotoxic effects and wound healing activity of ternary composite substance (hollow silica sphere/gum arabic/methylene blue)

Duran H., Alkan F. U. , Ulkay M. B. , Karakus S. , Aktas A. , Sismanoglu T.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.121, ss.1194-1202, 2019 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 121
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.ijbiomac.2018.10.098
  • Sayfa Sayıları: ss.1194-1202


We aimed to test the adsorption of the methylene blue (MB) on HSEPCGUM as a dye and also to test the obtained ternary biocomposite substance (HSEPCGUM-MB) on wound healing. Hollow silica spheres (HSS) are used in the pharmaceutical and biochemical field, because of low toxic, highly biocompatible and mechanically stable by large surface areas. HSS was obtained by mechanochemistry method. The obtained HSS was treated with epichlorohydrin to carry out an epoxidation process (HSEPC). Then, HSEPC was functionalized by treatment with gum arabic (HSEPCGUM). MB was adsorbed onto HSEPCGUM and the adsorption maximum capacity of HSEPCGUM (X-max) was obtained 333 mg g(-1). For in vitro studies, according to the cytotoxicity test results, ternary biocomposite substance (HSEPCGUM-MB) was studied at non-cytotoxic concentrations 10, 50 and 100 mu g/ml and wound closure was found as 55% (100 g/m1) as compared to control. (C) 2018 Elsevier B.V. All rights reserved.