In order to determine the usefulness of the pelvic lymphoscintigraphy in patients with metastatic lymph nodes involvement by gynecologic malignancies, a study was performed prio to operation and the findings were correlated with pathologic findings. The study was carried out in 5 subjects without and 25 patients with gynecologic malignancies. Of these patients, 17 had carcinoma of cervix uteri, 5 had carcinoma of corpus uteri and 3 had vulva cancer. For the scintigraphic investigation, 37 MBq of Tc-99m (Re) sulphide colloid in less than 1 ml were applied at each side. In 6 subjects, perianal injection and, in 24 patients, paracervical injection were done. All subjects also received bilateral pedal dorsal injection 3 hours after the first injection. Images were obtained in multiple projections at 3 and 6 hours after the first injections. All patients were operated on within the first five days after the scintigraphic evaluation. The sensitivity, specificity and accuracy were found as 100%, 77% and 82% respectively. In our study, the high sensitivity rate may be due to paracervical injection method which allows more apparent internal iliac lymph nodes visualization then perianal injection. In conclusion, paracervical injection in conjunction with bilateral pedal dorsal injection can be recommended in the preoperative assessment of lymph nodes involved by various gynecologic malignancies.