Tumor regressive effect of grape juice (Enoant®) on ehrlich ascites carcinoma of mice

Taskin E. I. , Yagci A., FIRAT Ü., CANER M. , Dogruman H.

Medycyna Weterynaryjna, cilt.63, sa.8, ss.926-930, 2007 (SCI Expanded İndekslerine Giren Dergi) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63 Konu: 8
  • Basım Tarihi: 2007
  • Dergi Adı: Medycyna Weterynaryjna
  • Sayfa Sayıları: ss.926-930


Enoant® is a nutritional reinforcement produced from the grape's stem, peel and seeds. In recent years grape products such as wine and grape juice have acquired a great importance because of their polyphenol components, which have a strong antioxidant effect. Those antioxidant effects of polyphenols have an ability to inhibit proliferation and induce apoptosis in cancer depending on the types of cancer and application doses. In our study we have aimed to investigate the tumor regressive effects of Enoant® in solid EAC tumor model in Balb-c mice. Animals were randomly divided into 2 groups in this study. 0.5 ml of Enoant® was administered daily to ENT group and the same volume of NaCl 0.9% was administered to the control group. Animals continued to receive those applications till sacrification day. On the 8th day 2 × 106 EAC cells in 0.5 ml NaCl 0.9% were injected subcutaneously into the mice's napes. On day 22 all animals were sacrificed under ether anesthesia. Using PCNA immunohistochemical staining, TUNEL technique, we observed the proliferative and apoptotic cell density changes in tumor tissues as well as the effect of Enoant® on these two phenomena. Dietary Enoant® significantly regressed tumor development in mice. It has been observed that the administration of Enoant® displayed positive effects on EAC tumor's weight and size when compared with control group animals. Mean tumor weights' meaningfulness was p < 0.01 and mean short-long diameters' meaningfulness were p < 0.05 and p<0.01, respectively. It has been determined that while the PCNA index was low (p < 0.05) in the Enoant® administered group, the apoptotic index that has been established with TUNEL technique was high (p < 0.01). As a result, Enoant® has a regressive function on EAC tumor cells. By inducing apoptosis, ENT inhibited the development of tumors. It is thought that ability of ENT was welded from its strong antioxidant polyphenol component. Because of that the use of Enoant® as a dietary supplement is thought to be a factor for inhibiting cancer development.