Tetracycline is a broad-spectrum antibiotic which is commonly used in humans and animals for treatment of bacterial infections. Therefore, tetracycline-resistant Enterobacteriaceae species found in the nature, humans, and animals are usually considered a serious health concern. The feces of birds that live with humans may be a source for of these antibiotic resistant bacteria. For this reason, presence of tetracycline resistant Enterobacteriaceae in bird droppings collected from 18 different breeders and pet shops fed in Istanbul was investigated by cultural and molecular methods in terms of the presence of that a/b gene. In addition, susceptibilities of isolates to various antibiotics were also determined. The current study reported that Enterobacter cloacae, Citrobacter freundi, Escherichia coli, Edwardsiella spp., Enterobacter spp., Escherichia vulneris, Klebsiella pneumonia, Pantoea agglomerans, Proteus mirabilis, Pantoe spp., Pseudomonas spp., Serratia marcescens, Salmonella spp., Shigella spp. were isolated from the samples. Ninety-seven of the isolates (97/150) were resistant to tetracycline (65.2%). In addition, the isolates were resistant to other antibiotic medications and 114 of them (58%) evaluated as multi drug resistant. The tet (A) and tet (B) genes were found in bacteria isolated from synantrophic birds' feces as 46.6% and 8%, respectively. Although working conditions are limited, the results obtained provide baseline information regarding antibiotic resistance to Enterocatericeae isolates obtained from captive birds' feces. Thus, the results of the present study emphasize the necessity of genotypic resistance research in future studies to help maintain antibiotic treatment efficacy for Enterocatericeae infections. Copyright 2018 Elsevier Inc. All rights reserved.