In vitro urease and trypsin inhibitory activities of some sulfur compounds

Dağsuyu E. , Yanardağ R.

İstanbul Journal of Pharmacy, cilt.51, sa.1, ss.85-91, 2021 (Hakemli Üniversite Dergisi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Konu: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.26650/istanbuljpharm.2021.844783
  • Dergi Adı: İstanbul Journal of Pharmacy
  • Sayfa Sayıları: ss.85-91


ABSTRACT Background and Aims: Organosulfur compounds modulate the activities of plurality of metabolic enzymes, especially those that activate (cytochrome P450s) or detoxify (glutathione-S-transferases) carcinogens. They also inhibit the formation of DNA adducts in different target tissues. The aim of the present study was to investigate the effect of some sulfur compounds on urease and trypsin activities in vitro. Methods: In the present study, the inhibitory effect of sulfur compounds on the activities urease and trypsin were determined according to the method of Hanif et al. (2012) and Ribeiro et al. (2010), respectively. Results: In comparison to the reference standard thiourea (IC50= 53.81±0.68 µg/mL), S-allyl-L-cysteine (IC50= 0.88±0.01 µg/ mL) and D, L-methionine (IC50= 0.91±0.02 µg/mL) had the highest urease inhibitor activity, corresponding to the lowest IC50 values among the sulfur compounds. Among the sulfur compounds used in this study, D,L-methionine (IC50= 0.13±0.01 mg/ mL) exhibited the lowest IC50 value for trypsin inhibitor, though its activity was less than that of tannic acid which was used as a standard (IC50= 0.06±0.01 mg/mL). Conclusion: The present outcome suggests that sulfur compounds are potential inhibitors of urease and trypsin activities, and may find importance in medicine and agriculture. Keywords: Enzyme, inhibitors, sulfur compounds, urease, trypsin