The efficacy of particleboards manufactured with sodium fluoride against mold fungi and Hylotrupes bajulus (L.) larvae was tested. Laboratory-size particleboards were manufactured from untreated wood particles with inclusion of fine sodium fluoride (NaF) at the 1, 1.5, and 3% levels relative to total particle weight. Sodium fluoride was introduced as powder during the manufacturing process just before blending resin with wood particles. The laboratory mold test indicated that even the lowest level of retention of NaF with or without leaching significantly reduced mold growth on particleboard surfaces when compared to the untreated control specimens. The increased retention of NaF from 1% to 3% further suppressed mold growth towards lowest levels even on leached specimens. The laboratory Hylotrupes bajulus larvae tests revealed that the particleboard environment with or without NaF is not appropriate for larvacidial activity. While the tested biocide, NaF, tested positive against mold growth on particleboard surfaces, no effect was determined relative to larval deaths.