The simultaneous electrochemical determination of multiple tumor antigens has attracted a great deal of attention, which can effectively enhance the capability and accuracy of the analysis. Nanostructured materials mostly played a key major role in the electrochemical immunosensors fabrication and operation improvement. This review focused mainly on the protocols for using nanostructures to fabricate electrochemical (nonlabeled@label-free and labeled@sandwich-type) immunosensors. Furthermore, this review has also described the diverse classes of electroactive nanospecies which are a complementary part of any immunosensor that assists to reach the selectivity for the target antigen. Finally, the important analytical characteristics of the published immunosensors were discussed (electrochemical detection technique, linear range, and detection limit). Studies published between the years 2009-2018 have been included in this review.