Effect of Different Thawing Time and High Temperature on Frozen Thawed Bull Semen Traits

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Yılmaz E., Ak K. , Baran A.

Journal of Animal and Veterinary Advances, cilt.18, ss.239-245, 2019 (ESCI İndekslerine Giren Dergi)

  • Cilt numarası: 18 Konu: 7
  • Basım Tarihi: 2019
  • Dergi Adı: Journal of Animal and Veterinary Advances
  • Sayfa Sayıları: ss.239-245


In this study was to investigate the effect of high temperature thawing and post-thaw cold shock

application on sperm motility as well as acrosomal and total abnormalities of frozen bull semen. Four Holstein

bulls were used to frozen semen in 0.25 mL straws. Semen of each bulls were thawed in 45 sec at 37°C (Group

A = Control group) in 15 sec at 50°C (Group B) and 5 sec at 70°C (Group C) and post-thawing cold shock

(300 sec at 5°C) were applied to all groups. After spermatozoa motility and morphological examinations are

performed sperm samples were incubated at 35°C for 120 min and spermatological traits were repeated. Semen

samples from the control and treatment groups were placed in an incubator taking into consideration the

medium conditions (Modified buffered Hepes medium) and the time needed for spermatozoa to reach the

fertilization site. Motility and morphological defects rates were determined with phase contrast microscopy and

motility rate and speed with sperm fertility analyser (SFA-500). The Hancock’s solution was used for

morphologic examination of spermatozoa (acrosome, other and total). In computer aided sperm fertility

analyser, motility and movement were performed in according to the technique. In conclusion, it has been found

that the conventional thawing method and rapid thawing technique are successful methods where the thawing

method applied to Group B damaged spermatozoon viability. The rate of thawing in Group B did not protect

the spermatozoa from dissolving or from the harmful effects of cold shock.