This research aimed to enrich the bioactive components of organic cherry laurel vinegar with ultrasound and to examine the correlation between artificial neural network (ANN) and response surface methodology (RSM), and compare some of the quality parameters of bioactive-enriched organic cherry laurel vinegar (UT-CLV), thermally pasteurized cherry laurel vinegar (P-CLV), and unprocessed organic cherry laurel vinegar (CLV). UT-CLV preserved and improved its volatile aroma profile more than the P-CLV sample. With ultrasound treatments, it provided positive effects on the antidiabetic and antihypertensive activities of organic cherry laurel vinegar. Also, organic cherry laurel vinegar was found to significantly inhibit the viability of prostate cancer cells, but not breast and lung cancer cells. As a result, it has been found that ultrasound technology enriches the bioactive components of organic cherry laurel vinegar according to thermal pasteurization and is successful in increasing its antidiabetic and antihypertensive effects. Novelty impact statement Bioactive components of organic cherry laurel vinegar were enriched with ultrasound treatment. Its antidiabetic, antihypertensive, and anticarcinogenic effects were found to be successful with ultrasound treatment. ANN and RSM had high prediction accuracy in different bioactive components.