Purpose To determine the knowledge and practices of nurses working in psychiatry clinics on the ventrogluteal (VG) injection. Design and Methods This study was conducted as a descriptive and cross-sectional study. A total of 91 nurses constituted the sample of the study. Findings It was determined that 35.7% of the nurses preferred the VG site. It was determined that the nurses who preferred the VG site knew and applied the preparation for injection, and the stages during and after the injection. Practice Implications This study will guide the preparation of in-service training programs on the use of VG site in psychiatric clinics.