Positioning stereotactic navigation system (PSNS) is a mechanical system to make a 3D coordinate metallic frame for guiding minor targets in the brain to do biopsy, injection where the treatment is needed. A PSNS head frame is fixed to skull by specially designed pins to keep the head from touching within the frame until the surgery completed. There are although complications in establishing a dependable frame of position, such as bone area that bear a fixed spatial liaison to soft tissues. The fixation or positioning techniques of head frame have been explained in various publications, but related research of mechanical stress/strain analysis about PSNS and pins has not been reported in literature. As a result, the study was undertaken to explore the PSNS head frame and pins by commercial CAD/CAM and ANSYS software packages. The research aim was to develop a three dimensional finite element model and von-Mises criteria model to calculate the mechanical behavior response for the stress distribution around PSNS and related material involving physical surface contact flexural and compressive loading mechanisms. Static analyses were carried out to find the generated stresses and deformation on each part of the studied model. The outcomes reveal that the finite element engineering applied to PSNS model may be employed to calculate the regional distribution of stress-strain-deformation growth in the skull. The knowledge gained from the study points toward some of the engineering advances that are beneficial to stereotactic frame design and mechanical engineers.