Isolation and preparation of monoclonal antibody to ovine Immunoglobulin M


Yilmaz H. , Tantas A., Ilgaz A., Morgan K.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.23, ss.135-140, 1999 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 23 Konu: 2
  • Basım Tarihi: 1999
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Sayfa Sayıları: ss.135-140

Özet

Ovine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.