Recently metal nanoparticles have become increasingly attractive adsorbents for the isolation of various compounds. In this regard, this investigation aims to evaluate the removal performance of copper oxide (CuO) nanoparticles as a novel adsorbent for tartaric acid from aqueous solution. For this aim, this study presents the preparation, characterization, and adsorption properties of CuO nanoparticles for the isolation of tartaric acid. CuO nanoparticles were synthesized by utilizing a simple procedure and characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and Brunauer-Emmett-Teller (BET) surface area. Afterwards, the removal properties of CuO nanoparticles were assessed under various adsorption conditions. CuO nanoparticles exhibited the highest removal performance with a higher adsorption capacity value of 619.23 +/- 2.08 mg g(-1). The Freundlich isotherm model was achieved to be the most accurate model with an R (2) value of 0.9533. Moreover, the pseudo-second order model was the most favorable to the kinetic measurements with an R (2) value of 0.9967. The results obtained in this study indicate that CuO nanoparticles have good removal properties for tartaric acid and may be employed as an efficient novel adsorbent for its isolation from the aqueous solution and wastewater.