The total selenium levels of 335 water samples of Turkey were determined by a spectrofluorometric method. The samples were digested in nitric-perchloric acid mixture, potential interferences were masked with disodium EDTA-HONH2. HCl and selenium was complexed with freshly prepared 2,3-diaminonaphthalene solution and estimated spectrofluorometrically after extraction in cyclohexane. The selenium content of various waters (rain, tap, mineral, sea, lake, river, bottled drinking waters and collected drinking waters from 42 cities in Turkey) were determined. The selenium levels were compared with the literature data from different countries.