Relationship between DNA damage and total antioxidant capacity in patients with glioblastoma multiforme


Tüzgen S. , Hanımoğlu H. , Tanrıverdi T. , Kaçıra T. , Sanus G. Z. , Atukeren P. , ...More

CLINICAL ONCOLOGY, vol.19, no.3, pp.177-181, 2007 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1016/j.clon.2006.11.012
  • Title of Journal : CLINICAL ONCOLOGY
  • Page Numbers: pp.177-181

Abstract

Aims: To assess oxidative DNA damage and total antioxidant capacity (TAC) in glioblastoma multiforme (GBM) and to compare the results with normal brain tissues. Materials and methods: Oxidative DNA damage and TAC were evaluated in GBM tissues extracted from 26 patients and in normal brain tissues of 15 subjects who underwent autopsy within the first 4 h of death. Oxidative DNA damage was assessed by measuring 8-hydroxy-2-deoxyguanosine (8-OH-dG) using the 8-OH-dG enzyme immunoassay kit, a quantitative assay for 8-OH-dG, and TAC was analysed using the ImAnOx colorimetric test system for the determination of antioxidative capacity. The results were compared between two groups and any correlation between 8-OH-dG and TAC was sought. Results: The median level of TAC in GBM (121.5 nmol/g wet tissue) was remarkably lower than that in normal brain tissue (298 nmol/g wet tissue). The difference was statistically significant (P = 0.00001). In contrast, oxidative DNA damage was significantly higher in patients with GBM (74.9 ng/g wet tissue) than in controls (34.71 ng/g wet tissue). Again, the difference was statistically significant (P = 0.00001). We also found a negative correlation between oxidative DNA damage and TAC (P < 0.001). Conclusions: These findings indicate that the degree of oxidative DNA damage is increased and TAC is decreased in GBM. Oxidative DNA damage is correlated with the levels of TAC.