Protein carbonyl content in erythrocyte membranes in type 2 diabetic patients


Konukoglu D. , Kemerli G., SABUNCU T., HATEMİ H.

HORMONE AND METABOLIC RESEARCH, vol.34, no.7, pp.367-370, 2002 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 7
  • Publication Date: 2002
  • Doi Number: 10.1055/s-2002-33468
  • Title of Journal : HORMONE AND METABOLIC RESEARCH
  • Page Numbers: pp.367-370
  • Keywords: lipid peroxidation, protein oxidation, protein carbonyl, diabetes mellitus, angiopathy, OXIDATIVE STRESS, DAMAGE, PLASMA, COMPLICATIONS, GLYCATION, MELLITUS, CELLS

Abstract

Protein carbonyl groups result from free radical-induced protein oxidation; their level in tissues and plasma is a relatively stable marker of oxidative damage. Protein carbonyl contents in erythrocyte membranes were investigated in the type 2 diabetic patients with good (n = 16) and poor (n = 30) glycemic control. Diabetic patients were classified as patients with (n = 20) and without (n = 26) angiopathy. Protein carbonyl content was evaluated using the 2,4-dinitro-phenyl-hydrazine method. Protein carbonyl content and GHb levels were significantly higher in both patients with poor and good glycemic control than in control subjects (p < 0.001 in each case). There was a significant difference in protein carbonyl content between patients with poor and good glycemic control (p < 0.001). Diabetic patients with angiopathy had significantly higher protein carbonyl content and GHb levels than the diabetic patients without angiopathy (p < 0.001). These results suggest that impaired glycemic control is connected to protein oxidation, and protein oxidation may be related to underlying metabolic abnormalities and complications of diabetes.