© 2020, Veteriner Fakultesi Dergisi. All rights reserved.Hibernoma is a very rare benign tumor of brown fat tissue which is found in hibernating and non-hibernating mammals. Until now, it has been reported in rats, dogs, and human beings. In this case report, a thirteen-year-old, spayed, female Siamese cat was examined for intestinal motility disorder. Local obstruction of the colon descendens was observed on radiographs 72 h after contrast medium administration. In order for the abdominal organs to be examined, diagnostic laparotomy was performed. A mass 1 cm in diameter located on the abdominal wall showing adhesion to mesenterium of the colon descendens was observed and completely removed. Post-operative treatment was maintained by intestinal diet, metoclopramide, and enrofloxacin administration. Histopathological examination of the mass revealed hibernoma, a benign tumor of brown fat tissue. Further immunohistochemical analyses were performed to evaluate the origin and behavior of the tumor by using S-100 and osteopontin antibodies. As a result, immunohistochemical staining was positive for S-100 and was weakly positive for osteopontin antibodies. In the presented report, a case of hibernoma in a cat was described with the clinical, histopathological, immunohistochemical findings and treatment procedure.