OBJECTIVES In this study, the aim was to perform quality control of the Prowess Panther treatment planning system by comparing with the measurement data. METHODS For dosimetric measurements, Iba brand cc13 and FC-65P 0.6cc model (Scanditronix, Wellhöfer, Germany), the cylindrical ion chambers, and Iba brand dose1 electrometer model, RW3 solid water phantom and RFA-300 water phantom, were used. Measurements were performed with Elekta Synergy linear accelerator. RFA-300 Wellhöfer profile measurements were taken using a water phantom in the wedge and open field depth dose, off-axis ratio, and different x, y and diagonal directions. Output measurements were performed with RW3 solid water. RESULTS Open and wedge field percentage depth dose (PDD) and profiles of the treatment planning system were compared with measurements at Dmax, 5 cm, 10 cm, and 15 cm depths. The results were within the boundaries of acceptable BJR and found less than 3%. CONCLUSION Based on the quality control results, the Prowess Panther v5.01 Planning System was within the boundaries of acceptable accuracy in dosimetry. © 2012 Association of Oncology.